Absolute counts of PB mature lineages and BM HSPCs subpopulations in healthy subjects across aging and their association with CH-related mutations. (A–G) Correlation between the absolute count of each cell subtype (expressed as cells/μl) in the PB and the age of the 56 subjects analyzed. Statistical test: Spearman r; monocytes, P = 0.011; PMN (polymorphonucleated cells), P = 0.491; DCs, P = 0.0011; NK, P = 0.0002; NKt P = 0.0086; B cells, P < 0.0001; T cells, P < 0.0001. (H–O) Correlation between the absolute count of each cell subtype (expressed as cells/μl) in the BM and the age of the 73 subjects analyzed. Statistical test: Spearman r; CD34⁺ cells, P = 0.0002; PreB/NK, P < 0.0001; ETP (early T progenitors), P = 0.806; GMP (granulocyte-monocyte progenitors), P < 0.0001; CMP (common myeloid progenitors), P = 0.005; MEP (megakaryocyte-erythrocyte progenitors), P = 0.014; EP (erythrocyte progenitors), P = 0.014; MKp, (megakaryocyte progenitors), P = 0.088. (P) CH-associated mutations analysis in aged BM samples (n = 8) via whole-exome sequencing. (Q–U) Absolute quantification of each cell subtype (expressed as cells/μl) in the BM of WT (n = 5) and CH-associated mutations (n = 3) donors. * < 0.05; ** < 0.01; *** < 0.001; **** < 0.0001.