Time-course analyzis of effet of CDK7 inhibition on A6-MCI cells and Xenopus embryos. (A) Confocal pictures of A6-MCI cells treated with DMSO (control), LDC4297, or YKL-5-124 at the indicated hpi and immunostained for centrin (centrioles), Ac-α-Tub (cilia), and ZO1 (tight Junctions) at 72 hpi. Graphs show quantification of the phenotype. DMSO n = 508; LDC4297 n = 452 (8 hpi), 492 (16 hpi), 530 (24 hpi), and 502 (48 hpi); and YKL-5-124 n = 494 (8 hpi), 527 (16 hpi), 526 (24 hpi), and 622 (48 hpi) cells. SEM is shown. Data were collected from three independent experiments. Fisher’s exact test: P < 2.2e−16 (***) for all comparisons. (B) Confocal pictures of Xenopus epidermis cells treated with DMSO (control) or YKL-5-124 at the indicated embryonic stages and immunostained for centrin, Ac-α-Tub, and ZO1 at stage 27. Graphs show quantification of the phenotype. DMSO n = 125 (DMSO) and YKL-5-124 n = 124 (St10), 141 (St12), and 160 (St20) cells. SEM is shown. Data were collected from three independent experiments. Fisher’s exact test: P < 2.2e−16 (***) for DMSO vs YKl-5-124 (St10), YKl-5-124 (St12) vs YKl-5-124 (St20); P = 1 (ns) for YKl-5-124 (St10) vs YKl-5-124 (St12).