Proteomic analysis of A6-MCI cells. (A) PCA analysis of the complete the proteomic dataset. (B–F) Volcano plots of differential expression analysis between the indicated times after induction. Log2 difference in expression between time points and −log P value are plotted. Upregulated proteins with a Log2 difference of >0.5 are shown in purple. Downregulated proteins with a Log2 difference less than −0.5 are shown in green. Ni vs 8 h: 125 up, 194 down; 8 vs 16 h: 222 up, 137 down; 16 vs 24 h: 301 up, 124 down; 24 vs 48 h: 699 up, 270 down; 48 vs 72 h: 187 up, 298 down. (G and H) Top 20 GO term processes associated with 16–24 h (G) and 24–48 h (H) transitions. (I) Graph showing the variation of expression of all Cdks detected in the proteomic dataset using the LFQ intensity. PCA, principal component analysis.