Deuterosome organization in A6-MCI cells. (A–D) Confocal pictures of 16 hpi A6-MCI (A, C, and D) or Xenopus epidermis (B) MCCs stained with the indicated deuterosome (Deup1, pericentrin, and γ-tubulin) or centriole (centrin) markers. (E–G) Expansion microscopy confocal picture of short (E), long (F), and ramified (G) A6-MCI deuterosomes stained for Deup1 and centrin. (H) STED super-resolution microscopy picture of globular A6-MCI deuterosomes stained with pericentrin and centrin. (I and J) TEM pictures of A6-MCI deuterosomes. J picture is duplicated in Fig. S4 B. (K and L) Segmentation obtained from tomogram acquisition of ramified (K) and short (L) A6-MCI deuterosomes. Scale bar: 2.5 μm (A–D); 280 nm (E–H); 225 nm (I and J).