Basal bodies in A6-MCI cells. (A–C) Confocal pictures of basal bodies of expanded differentiated A6-MCI cells stained for Ac-α-Tub (microtubules) and centrin (centriole lumen) (A), γ-tubulin (basal foot) and Cep164 (distal appendages) (B), or Cep164 and centrin (C). (D) Average map distribution of indicated markers. (E–H) TEM pictures from differentiated A6-MCI cells. (E) Lateral view of a basal body. (F) Top view of a basal body displaying 9 microtubule triplets and a basal foot nucleating individual cytoplasmic microtubule. (G) Top view of a basal body displaying 9 microtubule triplets and distal appendages. (H) View of basal bodies showing fan-shaped or long rootlets. (I and J) Confocal pictures of mature A6-MCI cells apical surface stained for β-tubulin (microtubules) and centrin (centrioles). (K and L) Confocal pictures of mature A6-MCI cells apical surface stained for actin and centrin (centrioles). (M and N) Confocal pictures of 72 hpi A6-MCI (M) and X. laevis epidermal MCCs (N) stained for centrin (centriole) and γ-tubulin (basal foot) reveal the polarity of basal bodies. (O) Quantification of basal body circular SD (CSD) in A6-MCI and X. laevis epidermal MCCs. Mean and SD are shown. n = 10 cells per condition. Data collected from one experiment. Unpaired t test with Welch’s correction: P < 0.0001 (****). Scale bar: 250 nm (A–C); 150 nm (E–G); 200 nm (H); 1 μm (I–L); 5 μm (M–O).