A6-MCI cells recapitulate the main differentiation steps of MCCs. (A–I) Confocal pictures of NI (A) or induced A6-MCI cells (B–I) at indicated hpi stained for the indicated markers. (J) Bar graph quantification of non-differentiated cells and differentiated cells at the indicated times after induction. For the stages NI, 8 h, and 16 h, cell differentiation was evaluated by the presence of Deup1 and centrin labelling. For the stages 24 h, 48 h, and 72 h, cell differentiation was evaluated by the presence of centrin labelling. Error bars are SD. n = 414 (NI), 456 (8 h), 293 (16 h), 288 (24 h), 478 (48 h), and 373 (72 h) cells. Data were collected from three independent experiments. (K) Bar graph showing the distribution of differentiated cells at 16 hpi, 24 hpi, and 72 hpi in deuterosome, centriole released, and ciliated categories evaluated by Deup1/centrin (see Fig. S3 C) and centrin/Ac-α-Tub staining, respectively. Error bars are SD. n = 115 (16 h), 288 (24 h), 148 (48 h), and 330 (72 h) cells. Data were collected from three independent experiments. Scale bar: 30 μm (A–I); 2.5 μm (E–G, inset).