Effects of dexamethasone concentration and application time on the differentiation of A6-MCI cells. (A) Confocal pictures of A6-MCI cells induced with variable concentrations of dexamethasone, applied at variable times and cultured for variable durations, as indicated, and stained for centrin (centrioles), Ac-α-Tub (cilia), and ZO1 (tight junctions). (B) Confocal pictures of NI A6-MCI cells stained for centrin, Ac-α-Tub and ZO1 after 72 h or 1 week of culture. (C) Graph showing the percentage of cells with multiple centrioles at 72 hpi for 8-h stimulation with increasing dexamethasone concentrations. n = 287 (1 nM), 418 (10 nM), 501 (100 nM), and 439 (1 μM) cells. SD between field of view is shown. Data were collected from one experiment. (D) Graph showing the percentage of cells with multiple centrioles at 72 hpi for 24-h stimulation with increasing dexamethasone concentrations. n = 936 (1 nM), 1277 (10 nM), 1432 (100 nM), and 1282 (1 μM) cells. SD between field of view is shown. Data were collected from one experiment. (E) Graphs showing the percentage of cells with multiple centrioles and with multiple cilia at 1 wk after induction for 24-h stimulation with increasing dexamethasone concentrations. n = 1111 (1 nM), 1253 (10 nM), 1483 (100 nM), and 1379 (1 μM) cells. SDs between experiments are shown. Data were collected from three independent experiments.