MCI expression in A6 cells drives differentiation in MCCs. (A–D) Confocal pictures of RPE1 (A), NIH3T3 (B), and A6 (C and D) cells transiently transfected with Myc-mMCIDAS or XMCIDAS-hGR and immunostained for the indicated markers. (E and F) Confocal pictures of stably transfected A6-MCI cells stained for the indicated markers. (G) Quantification of centriole number per cell in A6-MCI cells induced with increasing concentrations of dexamethasone and in X. laevis epidermal MCCs. Mean and SD are shown. n = 91 cells (1 nM), 124 cells (10 nM), 80 cells (100 nM), 103 cells (1 μM), and 155 cells (X. laevis). Data were collected from one experiment. (H) Percentage of A6-MCI cells with multicilia among those with multiple centrioles. Error bars are SD. n = 936 (1 nM), 1277 (10 nM), 1432 (100 nM), and 1282 (1 μM) cells. Data collected from two experiments. Scale bar: 10 μm (A–D), 13 μm (E and F).