Panel A: A scatter plot shows the relative eIF3e protein levels in control and cKO B cells at different time points of in vitro activation. The x-axis represents different time points (naive B, iGCB D1, iGCB D2, iGCB D3, iGCB D4), and the y-axis represents the relative eIF3e protein level. Each symbol represents an individual mouse, with small horizontal lines indicating the mean and standard deviation. Panel B: Another scatter plot shows the percentage of YFP+ cells in control and cKO B cells at the same time points. The x-axis represents the time points, and the y-axis represents the percentage of YFP+ cells. Each symbol represents an individual mouse, with small horizontal lines indicating the mean and standard deviation. Statistical significance is indicated similarly. Panel C: Flow cytometry plots show the analysis of cell death in control and cKO B cells at the iGCB D3 time point. The x-axis represents 7-AAD, and the y-axis represents Annexin 5. The percentages of cells in different quadrants are indicated. Panel D: Multiple flow cytometry histograms show the expression of various cell surface proteins (CD80, OX40L, ICOSL, ICAM-1, MHC II, GL7, CD40, CD70, CD86) in control and cKO B cells at different time points. The x-axis represents the fluorescence intensity, and the y-axis represents the percentage of maximum. Control and cKO groups are represented by black and red lines, respectively. Panel E: Bar graphs show the Metascape analysis of genes with upregulated (top) and downregulated (bottom) translation efficiency. The x-axis represents the gene count, and the y-axis represents the biological processes. The color scale indicates the -Log10 (P-value).
Analysis of cKO B cells upon in vitro activation. (A–D) Statistical analyses of the eIF3e protein level (A), percentage of YFP+ cells (B), FACS analysis of cell death (C), and cell surface protein expression (D) in control and cKO B cells at indicated time points of in vitro activation (Fig. 4 A). The representative FACS histograms in panel D (column 4) for the iGCB day 3 time point are also shown in Fig. 4, F and G. Each symbol in A and B represents an individual mouse. Small horizontal lines indicate the mean (±SD). Statistical significance is calculated using paired two-tailed Student’s t test (A and B). ns, not significant, P > 0.05; *P < 0.05; **P < 0.01. Three independent experiments (A–D) were performed. (E) Metascape analysis of genes highlighted in Fig. 4 I with upregulated (top) and downregulated (bottom) translation efficiency. Source data are available for this figure: SourceData FS3.