Panel A shows two scatter plots with age in weeks on the x-axis and cell counts in millions on the y-axis, comparing cluster of differentiation 8-positive T cells and other immune-cell populations between control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. Panel B includes scatter plots with age in weeks on the x-axis and percentages or counts of B-cell populations on the y-axis, showing differences between control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. Panel C features scatter plots with age in weeks on the x-axis and percentages or counts of plasma cells on the y-axis, comparing control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. Panel D presents scatter plots with age in weeks on the x-axis and percentages or counts of cluster of differentiation 4-positive T cells on the y-axis, highlighting differences between control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. Panel E includes scatter plots with age in weeks on the x-axis and percentages or counts of activated cluster of differentiation 4-positive T cells on the y-axis, comparing control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. Panel F shows an immunoblot analysis of eukaryotic translation initiation factor 3 subunit E expression in yellow fluorescent protein-negative and yellow fluorescent protein-positive B cells from 8-month-old conditional-knockout mice, with bands for eukaryotic translation initiation factor 3 subunit E and beta-actin loading control. Panel G displays flow-cytometry analysis of cluster of differentiation 8-positive T cells in spleens of 16-week-old control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice, with representative fluorescence-activated cell-sorting plots and scatter plots showing percentages and numbers of indicated T-cell populations.
Percentage, number, and activation status of T and B cells in cKO mice. (A–E) Percentage and number of T, B, and other immune cell subsets in the spleen of control and cKO mice at indicated ages. (F) Immunoblot analysis of eIF3e expression in YFP− and YFP+ B cells from 8-mo-old cKO mice. (G) Flow cytometry analysis of CD8+ T cells in the spleen of 16-wk-old control and cKO mice. Left: Representative FACS plots. Right: Number and percentage of indicated T cell populations. Each symbol represents an individual mouse. Small horizontal lines indicate the mean (±SD). ns, not significant, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, determined by two-tailed unpaired Student’s t test. Two independent experiments (A–G) were performed. Source data are available for this figure: SourceData FS1.