Panel A. Representative photographs compare spleen, peripheral lymph nodes, and mesenteric lymph nodes from control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice at different ages. Panel B features two scatter plots showing splenic B-cell and cluster of differentiation 4-positive T-cell numbers in control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice at different ages. The x-axis represents age in weeks, and the y-axis represents cell numbers. Panel C includes two scatter plots displaying the percentage and number of yellow fluorescent protein-positive B cells in spleens of control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice at different ages. The x-axis represents age in weeks, and the y-axis represents percentage and number of yellow fluorescent protein-positive B cells. Each symbol represents an individual mouse, with small horizontal lines indicating mean plus or minus standard deviation. Panel D presents flow-cytometry plots analyzing B cells in spleens of 16-week-old control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. The plots are gated from yellow fluorescent protein-positive B cells and show Fas cell-surface death receptor, GL7 germinal-center marker, cluster of differentiation 138, and immunoglobulin D expression. Panel E shows flow-cytometry plots analyzing cluster of differentiation 4-positive T cells in spleens of 16-week-old control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. The plots are gated from cluster of differentiation 4-positive T cells and show cluster of differentiation 62 ligand, cluster of differentiation 44, programmed cell death protein 1, and C-X-C motif chemokine receptor 5 expression. Panel F presents flow-cytometry plots analyzing interleukin-4 and interferon-gamma production by cluster of differentiation 4-positive T cells in spleens of 16-week-old control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice. Panel G shows immunofluorescence images of peripheral lymph nodes and spleens from 16-week-old control and eukaryotic translation initiation factor 3 subunit E conditional-knockout mice stained for cluster of differentiation 4, B220, and GL7. Scale bars represent 500 micrometers.
Eif3e deficiency causes lymphoproliferation. (A) Representative photos of the spleens, pLNs, and mLNs from control (Cγ1CreRosa26-YFPLSL) and cKO (Eif3efl/flCγ1CreRosa26-YFPLSL) mice at indicated ages. Scale bar, 1 cm. (B and C) Numbers of B and CD4+ T cells (B), and percentage and number of YFP+ B cells (C) in the spleen of control and cKO mice at indicated ages. Each symbol represents an individual mouse. Small horizontal lines indicate the mean (±SD). ns, not significant, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001, determined by two-tailed unpaired Student’s t test. (D–F) Flow cytometry analysis of B and CD4+ T cells in the spleen of 16-wk-old control and cKO mice. (G) Immunofluorescence analysis of pLN and spleen from 16-wk-old control and cKO mice. Scale bars, 500 μm. Two (A–F) and three (G) independent experiments were performed. pLNs, peripheral lymph nodes; mLNs, mesenteric lymph nodes.