Figure S5.
Confocal microscopy images showing mitochondrial degradation after wounding. Panel A shows confocal microscopy images of MAI-1 marked mitochondria cleared normally in mutant backgrounds. Panel B shows time lapse confocal microscopy images of MAI-1 and lysosomal marker recruitment after wounding. Panel C shows time lapse confocal microscopy images of lysosomal engulfment surrounding MAI-1 marked damaged mitochondria.

MAI-1–marked mitochondria are subject to lysosomal degradation. (A) Representative confocal images of MAI-1::GFP at 5 min and 24 h after wounding in pink-1 and pdr-1 mutant backgrounds. Clearance proceeded normally. Strains: pink-1(ok3538), pdr-1(gk448), and Pcol-19-MAI-1::GFP (zjuSi510). Scale bar, 10 and 5 μm (zoom in). (B) Representative time-lapse confocal images of MAI-1::GFP and NUC-1::mCherry during 1–60 min after laser wounding. Strain: Pced-1-NUC-1::mCherry (qxIs257); Pcol-19-MAI-1::GFP (zjuSi510). Scale bar, 10 μm. The white dashed circle indicates the wound site. (C) Representative time-lapse confocal images of MAI-1::mKate2 and SCAV-3::GFP before and at different time points after laser wounding. Strains: Pscav-3-SCAV-3::GFP (qxIs430); Pcol-19-MAI-1::mKate2 (zjuSi594). Scale bar, 5 μm. The white dashed circle indicates the wound site. The white arrowhead indicates enveloping event. (A–C): UW, unwounded; W, wounded.

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