Figure S4.
Panel A shows confocal images of MAI-1 fused to GFP and TOMM-20 fused to mKate2 under control, Oligomycin, and Rotenone treatments. The images are divided into three columns: MAI-1 fused to GFP, TOMM-20 fused to mKate2, and merged views, with zoomed-in sections for detailed observation. Panel B displays confocal images of MAI-1 fused to GFP and TMRE under the same conditions, also divided into three columns: MAI-1 fused to GFP, TMRE, and merged views, with zoomed-in sections.
Mitochondrial depolarization is insufficient to trigger MAI-1 translocation. (A) Representative confocal images of MAI-1::GFP and TOMM-20::mKate2 after oligomycin (10 μM, 2 h) or rotenone (100 μM, 2 h). Strain: Phyp-7-TOMM-20::mKate2 (zjuSi126); Pcol-19-MAI-1::GFP (zjuSi510). Scale bar, 10 and 5 μm (zoom in). (B) Representative confocal images of MAI-1::GFP and TMRE after oligomycin (10 μM, 2 h) or rotenone (100 μM, 2 h). TMRE was diminished, but MAI-1 remained cytosolic after 2 h of oligomycin and rotenone treatment. Strains: Pcol-19-MAI-1::GFP (zjuSi510). Scale bar: 10 and 5 μm (zoom in).