Figure 1.
A multi-panel image of mitochondrial markers before and after laser wounding. Panel A shows time lapse confocal microscopy images of mitochondrial changes after laser injury. Panel B shows time lapse confocal microscopy images of mitochondrial protein redistribution near wound regions. Panel C shows time lapse confocal microscopy images of membrane permeabilization following laser induced damage. Panel D shows super resolution microscopy images of disrupted mitochondrial structures after laser wounding.

Laser injury induces mitochondrial membrane permeabilization. (A) Representative time-lapse confocal images of mito::GFP and TMRE before and after laser wounding. Strain: Pcol-19-mito::GFP (zjuSi197). Scale bar, 10 μm. (B) Representative time-lapse confocal images of TOMM-20::mKate2 and mito::GFP before and after laser wounding. Strain: Phyp-7-TOMM-20::mKate2 (zjuSi126); Pcol-19-mito::GFP (zjuSi197). Scale bar, 10 μm. (C) Representative time-lapse confocal images of ATP-3::GFP and TMRE before and after laser wounding. Strain: ATP-3::GFP (KI, zju133). Scale bar, 10 µm. (D) Representative single-plane HIS-SIM images of ATP-3::GFP and TOMM-20::mYonghong before and after laser wounding. ROI, 1660 × 1660 pixels. Strains: ATP-3::GFP(zju133); Pcol-19-TOMM-20::mYonghong (zjuSi577). Scale bar, 10 and 1 µm (zoom in). The arrowheads indicate disruption of TOMM-20::mYonghong and ATP-3::GFP signals. (A–D) The white dashed circle indicates the wound site. UW, unwounded; W, wounded.

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