Figure S6.
Panel A shows western blot using anti-Nvj1 antibody comparing protein expression in wild type and nvj1 deletion strains during glucose starvation. Panel B shows western blot using anti-Nvj2 antibody comparing protein expression in wild type, nvj2 deletion, and Nvj2-GFP strains during glucose starvation. Panel C shows western blot using anti-Ypf1 antibody comparing protein expression in wild type and ypf1 deletion strains during glucose starvation. Panel D shows western blot using anti-Vac8 antibody comparing protein expression in wild type and vac8 deletion strains during glucose starvation. Panel E shows western blot using anti-Nsg1 antibody comparing protein expression in wild type, nsg1 deletion, and nsg1 deletion nsg2 deletion strains during glucose starvation. Panel F shows western blot using anti-Nsg2 antibody comparing protein expression in wild type, nsg1 deletion, and nsg1 deletion nsg2 deletion strains during glucose starvation.
Validation of antibody specificity used in this study. (A–F) Whole-cell lysates were prepared from the indicated cells under glucose-replete or glucose-starved (GS) conditions and analyzed by immunoblotting to validate the specificity of antibodies against Nvj1 (A), Nvj2 (B), Ypf1 (C), Vac8 (D), Nsg1 (E), and Nsg2 (F).