Panel A: Thin-layer chromatograms show lipid profiles of yeast cells cultured in YPD or GS medium with 14C-acetate. The chromatograms display bands corresponding to squalene, ergosterol ester, lanosterol, ergosterol, CL, PA, PE, PS, PI, and PC. Panel B: Bar graphs show that the x-axis lists different yeast strains, and the y-axis shows relative levels. Wild-type value is set to 1, and values are means with standard error (n = 3 or 4). Panel C: Thin-layer chromatogram shows bands corresponding to squalene, ergosterol ester, TAG, and ergosterol.
Squalene and ergosterol esters accumulate in an Hmg1-dependent manner in nsg1Δnsg2Δ cells. (A) The indicated yeast cells were cultured for 1 day in YPD or GS medium containing 14C-acetate, after which total lipids were extracted. The extracted lipids were treated with or without saponification, separated by thin-layer chromatography, and visualized by autoradiography. (B) Bar graph shows the combined levels of sterol lipids and squalene, normalized to phospholipid content. WT value was set to 1. Values are means ± SE (n = 3 or 4). (C) TAG levels were analyzed by TLC in WT, nsg1Δnsg2Δ, and lro1Δdga1Δ cells after 24 h of GS. Lipids were extracted and separated by TLC, and lipids were visualized by primuline staining. Source data are available for this figure: SourceData F6.