Figure S5.
The top row shows fluorescence microscopy images of Erg6-GFP, while the bottom row shows differential interference contrast (DIC) microscopy images. Each column represents a different yeast strain: wild-type (WT), nsg1 deletion, nsg2 deletion, nsg1 deletion nsg2 deletion, hmg1 deletion nsg1 deletion nsg2 deletion, hmg2 deletion nsg1 deletion nsg2 deletion, and atg1 deletion. The fluorescence images reveal the presence and distribution of Erg6-GFP, indicating lipid droplet accumulation. The DIC images provide a clear view of the cellular structure and the presence of lipid droplets within the cells. The scale bar in the DIC images represents 5 micrometers.
LDs accumulate in the absence of Nsg1 and Nsg2. WT and the indicated cells expressing Erg6-GFP were observed by confocal fluorescence microscopy after 4 days of GS. Maximum projection images reconstituted from z-stack are shown. Scale bars, 5 μm.