Figure S1.
Panel A shows a series of immunoblot image displaying protein bands at various molecular weights, indicated on the left in kilodaltons (kDa). The conditions are labeled at the top, including wild-type (WT), TurboID-HA, and CsFiND, with time points of 0, 1, 3, and 6 hours under control (mid-log) and nitrogen starvation. The proteins detected include SAv-Cy5, V5, FLAG, HA, and Tom70. Panel B presents a table summarizing protein scores from MASCOT analysis of LC-MS/MS data. The table lists proteins with their peptide numbers and scores under standard conditions (SCD) and nitrogen starvation (-N). The proteins include Faa1, Hmg2, Vac8, Nvj2, Nsg2, Hmg1, Nsg1, Nvj1, Tsc13, Mdm1, Lam6, Osh1, Ypf1, Cvm1, Nvj3, Pah1, Snd3, Vps13, and Ypt35.
Identification of NVJ-localized proteins using CsFiND. (A) WT yeast cells and yeast cells expressing CsFiND proteins or cytosolic TurboID-HA were cultured in SCD medium until logarithmic phase and then split into two halves. One is further cultivated in SCD medium containing biotin. Another half is shifted to SD-N medium containing biotin at a final concentration of 50 μM for the indicated hours. Total cell lysates were analyzed by streptavidin blotting or immunoblotting using the indicated antibodies. (B) Summary table showing protein scores obtained from MASCOT analysis of LC-MS/MS data for known NVJ proteins, as well as Ypf1, Nsg1, and Nsg2. Source data are available for this figure: SourceData FS1.