Figure 6.
Panel A: A schematic diagram shows the experimental setup for diphtheria toxin (DT) treatment in Cd11cCre+R26LSL-DTR mice to deplete alveolar macrophages (AMs) and monitor repopulation. Panel B: Flow cytometry plots illustrate the repopulation of AMs from recruited monocytes at days 1, 4, 7, and 10 post-DT treatment. The plots are gated on DAPI- CD45+ Ly6G- single cells, showing the expression of SiglecF and CD11c. Panels C to K: Bar graphs display the mRNA relative expression levels of various genes (Cdh1, Ctsk, Itgal, Mmp8, Spi1, Itgax, Slc9F, Ccr2, Itgam, Irf7, Rxra, Rxrb, Pparg, Csfr2b, Tgfbr1, Tgfb1, Notch1, Notch2, Csfr1, Csfr3r) in circulating monocytes (Circ Mon), recruited monocytes (Rec Mon), repopulating/differentiating cells at days 4-10 post-DT, and mature AMs. Each bar graph has the x-axis labeled with the cell types and the y-axis labeled with mRNA relative expression. Data points represent mean values with standard error of the mean (SEM), and statistical significance is indicated with asterisks.
Differentiating recruited monocytes increased expression of AM-specific genes and Rxra upon AM depletion. (A) DT treatment for AM depletion in Cd11cCre+R26LSL-DTR mice. (B) Representative flow cytometry plots illustrating AM repopulation from recruited monocytes after AM depletion. (C–K) Gene expression patterns in circulating monocytes (Circ Mon), recruited monocytes (Rec Mon), repopulating/differentiating cells at 4–10 days after DT, and mAMs, n = 3–5 mice per group. Data are representative of two independent experiments and presented as mean ± SEM; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001; one-way ANOVA.