Figure 2.
A multi-panel image showing cell proliferation and apoptosis in mice. Panel A shows a schematic timeline of daily BrdU injections followed by sacrifice and flow cytometry analysis in control and Cd11cCre positive Rxrab deficient mice. Panel B shows representative flow cytometry plots of BrdU incorporation in mature alveolar macrophages from control and Cd11cCre positive Rxrab deficient mice. Panel C shows a bar graph quantifying percentages of BrdU positive mature alveolar macrophages between genotypes. Panel D shows representative flow cytometry plots of Ki67 and DAPI staining used for cell cycle analysis in mature alveolar macrophages. Panel E shows bar graphs quantifying Ki67 positive mature alveolar macrophages distributed across G1, G2-S-M, and G0 cell cycle phases. Panel F shows representative flow cytometry plots of Annexin V labeling in mature alveolar macrophages from control and Cd11cCre positive Rxrab deficient mice. Panel G shows a bar graph quantifying percentages of Annexin V positive mature alveolar macrophages between genotypes. Panel H shows representative flow cytometry plots of Annexin V and BODIPY staining identifying lipid positive apoptotic mature alveolar macrophages in both genotypes. Panel I shows a bar graph quantifying percentages of Annexin V positive and BODIPY positive mature alveolar macrophages between genotypes.

RXR-depleted mAMs are more proliferative but have an exacerbated cell death rate linked to lipotoxicity. (A) Scheme of BrdU assay. (B) Representative flow cytometry plots showing BrdU incorporation in mAMs. (C) Quantification of BrdU+ mAMs. n = 8 per genotype; data are from two pooled independent experiments. (D) Representative flow cytometry plots showing Ki67/DAPI staining in mAMs. (E) Quantification of cell cycle distribution analysis in mAMs according to Ki67 and DAPI levels. n = 6–8 per genotype; data are from two pooled independent experiments. (F) Representative flow cytometry plots showing Annexin V labeling in mAMs. (G) Quantification of Annexin V+ mAMs. n = 6–8 per genotype; data are from two pooled independent experiments. (H) Representative flow cytometry plots showing BODIPY stain analysis in Annexin V+ mAMs. (I) Quantification of Annexin V+/BODIPY+ mAMs. n = 6–7 per genotype; data are from two pooled independent experiments. All data were obtained from 9-wk-old Rxrabfl/fl and Cd11cCre+Rxrabfl/fl mice and are shown as means ± SEM; analysis were performed based on FMO controls; ****P ≤ 0.0001; unpaired Student t test.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal

Gift article access

As a benefit of your subscription, you can share temporary access to restricted articles.

Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.

Please sign in to your personal account to gift article access.

Register

Gift article access

As a benefit of your subscription, you can share temporary access to restricted articles.

Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.

Gift articles remaining: --

Gift article access

Each link will stop working after 30 days or 10 uses. You may create up to 10 links in a 30 day period.

Gift articles remaining: --

Gift article access

As a benefit of your subscription, you can share temporary access to restricted articles.

Each link will stop working after 30 days or 10 uses.

You have reached the limit of 10 links within a 30 day period.

Sharing Unavailable

Sharing content requires targeting cookies to be enabled. Please update your cookie preferences to use this feature.