Figure 8.
The diagram shows four classes of adjacent transmembrane domains with inserting transmembrane domains shown in red and already inserted transmembrane domains shown in pink. The classes are labeled as transmembrane-domain 1 to 2 pair, internal pair with short loop, internal pair with long loop, and long outside loop. The violin plots display the apparent free energy change of membrane insertion in kilocalories per mole for different transmembrane-domain pairs. The horizontal axis lists categories: all transmembrane-domain pairs, transmembrane-domain 1 to 2, internal short-loop pairs, internal long-loop pairs, and internal long outside-loop pairs. The vertical axis ranges from minus 7.5 to positive 12.5 kilocalories per mole. Each violin plot shows the distribution of apparent free energy of membrane insertion values, with a box plot inside indicating the median, quartiles, and possible outliers. Summary statistics below the plots provide the number of instances and apparent free energy of membrane insertion values for transmembrane-domain 1, transmembrane-domain 2, and flanking regions.
Properties of TMDs separated by a short or long exoplasmic loop. Cartoons showing the four classes of adjacent TMDs that were analyzed, with the inserting two TMDs in red and already-inserted TMDs in pink. Violin plots for all TMD pairs and for each diagrammed class are shown along with summary statistics.