Figure 7.
Panel A: A schematic model and two violin plots compare the apparent free energy change of transmembrane domains that follow either a cleavable signal sequence or an Ncyt-long signal-anchor segment. The horizontal axis labels are signal sequence and Ncyt-long signal-anchor, while the vertical axis represents apparent free energy of membrane insertion in kilocalories per mole, ranging from minus 6 to positive 6. The signal-sequence plot shows a median apparent free energy of membrane insertion of approximately minus 1.89 kilocalories per mole with a 9.4 percent spread, while the Ncyt-long signal-anchor plot shows a median apparent free energy of membrane insertion of 0.00 kilocalories per mole with a 51.3 percent spread, while summary statistics include transmembrane-domain length, apparent free energy of membrane insertion, and flanking-charge properties. Panel B: Two violin plots compare apparent free energy of membrane insertion of transmembrane domains in single-pass and multipass proteins. The horizontal axis labels are single-pass and multipass, while the vertical axis represents apparent free energy of membrane insertion in kilocalories per mole, ranging from minus 6 to positive 6. The single-pass plot shows a median apparent free energy of membrane insertion of approximately minus 2.10 kilocalories per mole with a 9.4 percent spread, while the multipass plot shows a median apparent free energy of membrane insertion of approximately minus 0.80 kilocalories per mole with a 27.5 percent spread, while accompanying statistics summarize transmembrane-domain length and distributions of positively charged flanking residues.
Properties of TMDs that insert through Sec61. (A) Properties of TMDs that are downstream of either a cleavable SS or an Ncyt-long SA. Violin plots and summary statistics are shown below the diagrams. (B) As in A, but subdivided into single-pass and multipass proteins.