Figure 6.
Panel A: A schematic model and three vertical violin plots are shown. The horizontal axis labels the classes: Nexo (purple), Ncyt-long (green), and Ncyt-pair (yellow). The vertical axis measures apparent free energy of membrane insertion in kilocalories per mole, ranging from minus 7.5 to positive 12.5. Each violin plot shows the distribution of apparent free energy values for each class, with embedded box plots indicating the mean (diamond), median, and interquartile range. Summary statistics below the plots include the number of samples, length in amino acids, apparent free energy of membrane insertion, positively charged flanking residues on the cytosolic side, and positively charged flanking residues on the exoplasmic side, colored according to hydrophobicity and net-charge scales. Panel B: Four vertical violin plots compare the properties of signal-anchor transmembrane domains subdivided into single-pass and multipass groups. The horizontal axis labels the groups: Nexo single-pass, Nexo multipass, Ncyt-long single-pass, and Ncyt-long multipass. The vertical axis measures apparent free energy of membrane insertion in kilocalories per mole, ranging from minus 7.5 to positive 12.5. Each violin plot shows the distribution of apparent free energy values for each group, with embedded box plots indicating the mean (diamond), median, and interquartile range. Summary statistics below the plots include the number of samples, length in amino acids, apparent free energy of membrane insertion, positively charged flanking residues on the cytosolic side, and positively charged flanking residues on the exoplasmic side, colored according to hydrophobicity and net-charge scales.
Properties of SA TMDs. (A) Comparison of the properties of three classes of cotranslationally inserted SA TMDs: Nexo (purple), Ncyt-long (green), and Ncyt-pair (yellow). Violin plots are shown below the respective diagrams. In this and subsequent figures, the violin plots show ΔGapp distributions for each class, with embedded box plots indicating mean (diamond), median, and interquartile range. Summary statistics are shown below the violin plots, colored according to the heatmap scales for hydrophobicity and net charge under B. (B) As in A, but subdivided into SP and MP groups. SP, single pass; MP, multipass.