Figure S1.
Additional analyses of the prostate phenotype of SB mice (related to Fig. 2). (A) PCR showing evidence of excision of the T2/Onc2 transposon in induced NPp53-SB(+) tumors (lanes 3–8) but not in tumors without the SB (NPp53-SB(−)) or non-induced NPp53-SB(+) tumors (lanes 1, 2, respectively) (n = 10). (B and C) Analysis of the phenotype of NP-SB(−) (n = 12) and NP-SB(+) mice (n = 18). (B) Kaplan–Meier survival analysis. P value was calculated using a Log-rank test. (C) Representative H&E images of prostate tumors showing low power and high-power. n = 5 independent tumors/group. Scale bars represent 50 µm for low power and 20 µm for high power H&E. (D and E) Additional analyses of non-NEPC and NEPC tumors from the NPp53-SB(−) and NPp53-SB(+) mice. (D) Summary of tumor weights from NPp53-SB(−) (n = 29) and NPp53-SB(+) non-NEPC (n = 43) and NPp53-SB(+) NEPC (n = 27) mice. (E) Quantification of Ki67 expression in tumors from NPp53-SB(−) and NPp53-SB(+) non-NEPC and NEPC. Data were obtained by counting a minimum of 8,000 cells from five sections per tumor from three independent tumors per condition. (F and G) Analyses of metastasis in NPp53-SB(−) and NPp53-SB(+) mice. Representative cases of NPp53-SB(−) (n = 29) and NPp53-SB(+) non-NEPC (n = 43) and NPp53-SB(+) NEPC (n = 27) mice. (F) Representative H&E images of the indicated tissues. Scale bars represent 50 µm. (G) Metastasis frequency for the tissues indicated. (H–K) Representative H&E images showing examples of variant tumor histology for NPp53-SB(−) (n = 29) and NPp53-SB(+) non-NEPC (n = 43) and NPp53-SB(+) NEPC (n = 27) mice. (H and I) Selected NPp53-SB(−) cases. (J and K) Selected NPp53-SB(+) cases. Scale bars in H and J represent 50 µm for low power (top) and 20 µm for high power (bottom) images. Scale bars in I and K represent 250 µm for low power (left side) and 100 µm for high power (right side). Detailed histopathological analysis is provided in Table S1. Source data are available for this figure: SourceData FS1. Refer to the image caption for details. Panel A shows a polymerase chain reaction gel image with lanes indicating excision of the T2/Onc2 transposon. Panel B shows a Kaplan–Meier survival analysis line graph comparing NP-SB negative and NP-SB positive mice, with the y-axis representing survival probability in percent and the x-axis representing days after TAM. Panel C shows representative hematoxylin and eosin stained images of prostate tumors at low and high magnification for NP-SB negative and NP-SB positive mice. Panel D shows a violin plot summarizing tumor weights from NPp53-SB negative and NPp53-SB positive non-NEPC and NEPC mice, with the y-axis representing weight in grams. Panel E shows a box plot quantifying Ki67 expression in tumors, with the y-axis representing the percentage of Ki67-positive cells. Panel F shows representative hematoxylin and eosin stained images of lymph node, lung, and liver tissues. Panel G shows a bar graph depicting metastasis frequency in these tissues, with the y-axis representing percentage. Panels H through K show representative hematoxylin and eosin stained images of variant tumor histology for NPp53-SB negative and NPp53-SB positive non-NEPC and NEPC mice at different magnifications.

Additional analyses of the prostate phenotype of SB mice (related to Fig. 2). (A) PCR showing evidence of excision of the T2/Onc2 transposon in induced NPp53-SB(+) tumors (lanes 3–8) but not in tumors without the SB (NPp53-SB(−)) or non-induced NPp53-SB(+) tumors (lanes 1, 2, respectively) (n = 10). (B and C) Analysis of the phenotype of NP-SB(−) (n = 12) and NP-SB(+) mice (n = 18). (B) Kaplan–Meier survival analysis. P value was calculated using a Log-rank test. (C) Representative H&E images of prostate tumors showing low power and high-power. n = 5 independent tumors/group. Scale bars represent 50 µm for low power and 20 µm for high power H&E. (D and E) Additional analyses of non-NEPC and NEPC tumors from the NPp53-SB(−) and NPp53-SB(+) mice. (D) Summary of tumor weights from NPp53-SB(−) (n = 29) and NPp53-SB(+) non-NEPC (n = 43) and NPp53-SB(+) NEPC (n = 27) mice. (E) Quantification of Ki67 expression in tumors from NPp53-SB(−) and NPp53-SB(+) non-NEPC and NEPC. Data were obtained by counting a minimum of 8,000 cells from five sections per tumor from three independent tumors per condition. (F and G) Analyses of metastasis in NPp53-SB(−) and NPp53-SB(+) mice. Representative cases of NPp53-SB(−) (n = 29) and NPp53-SB(+) non-NEPC (n = 43) and NPp53-SB(+) NEPC (n = 27) mice. (F) Representative H&E images of the indicated tissues. Scale bars represent 50 µm. (G) Metastasis frequency for the tissues indicated. (H–K) Representative H&E images showing examples of variant tumor histology for NPp53-SB(−) (n = 29) and NPp53-SB(+) non-NEPC (n = 43) and NPp53-SB(+) NEPC (n = 27) mice. (H and I) Selected NPp53-SB(−) cases. (J and K) Selected NPp53-SB(+) cases. Scale bars in H and J represent 50 µm for low power (top) and 20 µm for high power (bottom) images. Scale bars in I and K represent 250 µm for low power (left side) and 100 µm for high power (right side). Detailed histopathological analysis is provided in Table S1. Source data are available for this figure: SourceData FS1.

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