Figure S2.
Shared and distinct roles of E- and N-cadherin in polarity development and BC formation in Can 10 cells, related to Fig. 3. (A) Quantification of total apical–basal polarity (combined hepatic and columnar polarity) in E- or N-cadherin knockdown cells. Data represent means ± SD from four independent experiments (≥480 cells per condition). (B) E- and N-cadherin jointly contribute to AJ assembly and apical–basal polarity. Shown are representative confocal images of Can 10 cells transfected with E- and N-cadherin siRNAs, cultured for 72 h, and stained with DAPI and antibodies against aPKC and N-cadherin. (C) Quantification of polarity and BC structures in double knockdown cells. Data represent means ± SD from four independent experiments (≥350 cells per condition). (D) Localization patterns of E- and N-cadherin during different stages of cytokinesis. Shown are representative confocal images of Can 10 cells cultured for 72 h, and stained with DAPI and the indicated antibodies. Arrowheads, arrows, and double arrows indicate AJs, polar cortex, and the basal cleavage furrow or midbody, respectively. Scale bars, 20 µm (B), 5 µm (D). P values are indicated at the top of each graph; n.s., not significant. Refer to the image caption for details. Panel A: Two vertical bar graphs show that the x-axis represents different siRNA conditions, and the y-axis represents the percentage of cells with polarity. The left graph shows data for E-cadherin knockdown, and the right graph shows data for N-cadherin knockdown. Both graphs indicate means and standard deviations from four independent experiments. Panel B: Confocal images display cells transfected with E- and N-cadherin siRNAs, stained with DAPI, aPKC, and N-cadherin antibodies. The images show the contribution of E- and N-cadherin to adherens junction assembly and apical-basal polarity. Panel C: Four vertical bar graphs show that the x-axis represents different siRNA conditions, and the y-axis represents the percentage of cells with specific polarity or BC structures. The graphs show means and standard deviations from four independent experiments. Panel D: Confocal images show the cells are stained with DAPI and various antibodies. Arrowheads, arrows, and double-arrows indicate adherens junctions, polar cortex, and the basal cleavage furrow or midbody.

Shared and distinct roles of E- and N-cadherin in polarity development and BC formation in Can 10 cells, related to Fig. 3. (A) Quantification of total apical–basal polarity (combined hepatic and columnar polarity) in E- or N-cadherin knockdown cells. Data represent means ± SD from four independent experiments (≥480 cells per condition). (B) E- and N-cadherin jointly contribute to AJ assembly and apical–basal polarity. Shown are representative confocal images of Can 10 cells transfected with E- and N-cadherin siRNAs, cultured for 72 h, and stained with DAPI and antibodies against aPKC and N-cadherin. (C) Quantification of polarity and BC structures in double knockdown cells. Data represent means ± SD from four independent experiments (≥350 cells per condition). (D) Localization patterns of E- and N-cadherin during different stages of cytokinesis. Shown are representative confocal images of Can 10 cells cultured for 72 h, and stained with DAPI and the indicated antibodies. Arrowheads, arrows, and double arrows indicate AJs, polar cortex, and the basal cleavage furrow or midbody, respectively. Scale bars, 20 µm (B), 5 µm (D). P values are indicated at the top of each graph; n.s., not significant.

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