Figure S4.
A set of histograms showing the distribution of phosphorylated STAT1 (pSTAT1) levels. The horizontal axis represents pSTAT1 levels, and the vertical axis represents the frequency of cells. The histograms are grouped into three rows: Patient 2 (rescued), Patient 2 (empty vector), and Healthy Control. Each group has two histograms, one for unstimulated conditions and one for stimulated conditions. A set of histograms showing the distribution of phosphorylated STAT3 (pSTAT3) levels. The horizontal axis represents pSTAT3 levels, and the vertical axis represents the frequency of cells. The histograms are grouped into three rows: Patient 2 (rescued), Patient 2 (empty vector), and Healthy Control. Each group has two histograms, one for unstimulated conditions and one for stimulated conditions (100 ng/ml OSM). A set of histograms showing the distribution of phosphorylated STAT5 (pSTAT5) levels. The horizontal axis represents pSTAT5 levels, and the vertical axis represents the frequency of cells. The histograms are grouped into three rows: Patient 2 (rescued), Patient 2 (empty vector), and Healthy Control. Each group has two histograms, one for unstimulated conditions and one for stimulated conditions.
WT OSMR restores STAT signaling in primary fibroblasts. Phosphorylation of STAT1, STAT3, and STAT5 was quantified in primary fibroblasts from P2 that were transduced with WT OSMR or an EV and compared to primary fibroblasts from an HC before and after treatment with OSM (100 ng/ml for 15 min); n = 3.