Figure S2.
Contour plots showing extracellular OSMR expression in HEK293 cells. The horizontal axis represents OSMR beta levels, and the vertical axis represents GFP levels. Wild-Type OSMR beta is shown in the first plot. FPLCA variants (p.Asn462Ser, p.Gly513Asp, p.Val631Leu, and p.Ile691Thr) are shown in the next plots. OSMR deficiency variants (p.Ala349Asp, p.Val436Asp, and p.Tyr660Serfs*16) are shown in the last three plots. Contour plots showing total OSMR expression in HEK293 cells. The horizontal axis represents OSMR beta levels, and the vertical axis represents GFP levels. Wild-Type OSMR beta is shown in the first plot. FPLCA variants (p.Asn462Ser, p.Gly513Asp, p.Val631Leu, and p.Ile691Thr) are shown in the next plots. OSMR deficiency variants (p.Ala349Asp, p.Val436Asp, and p.Tyr660Serfs asterisk 16) are shown in the last three plots.
Contour plots demonstrating loss of cell surface OSMRβ expression in HEK293 cells. Extracellular and total OSMRβ expression were quantified in HEK293 cells transfected with WT OSMRβ, OSMRβ LOF variants (p.Ala349Asp, p.Val436Asp, p.Gln51Thrfs*23, p.Gln270*, p.Pro478Hisfs*18, and p.Tyr660Serfs*16), or FPLCA variants (p.Asn462Ser, p.Gly513Asp, p.Val631Leu, and p.Ile691Thr) using flow cytometry.