Figure 3.
Panel A shows bar graphs for naive B cells, transitional B cells, unswitched memory B cells, CD21lo B cells, switched memory B cells, and plasmablasts. The x-axis represents patients P5, P6, and P8, while the y-axis represents cell counts per microliter. Panel B displays radar charts for patients P5, P6, and P8, showing the distribution of unswitched memory B cells, transitional B cells, CD21lo B cells, switched memory B cells, and plasmablasts before and after eculizumab treatment. The radar charts use different colors to represent pre- and post-treatment conditions. Panel C includes flow cytometry dot plots for patient P5, showing the distribution of B-cell subsets before and after eculizumab therapy. The dot plots use markers such as CD27, IgD, CD24, and CD38 to differentiate between various B-cell subsets.
B cell subset frequencies and counts before and after eculizumab therapy in patients P5, P6, and P8. (A and B) B cell subset distributions before and after eculizumab treatment are shown as bar graphs (A) and radar charts (B). Eculizumab treatment was associated with an increase in transitional B cell frequencies and absolute counts and a marked reduction in CD21lo B cells, switched memory B cells, and plasmablasts in all three patients. (C) These changes are further illustrated by FCM dot plots depicting the distribution of B cell subsets before and after eculizumab therapy in patient P5. sm, switched memory; unsm B cells, unswitched memory.