Figure 5. Panel A shows a steady state... | Rockefeller University Press

Figure 5.

$SSI behavior for NaV1.2_IQM expressed with different FGF-A homologues. (A) SSI protocols (identical to those in Fig. 2) were used to examine fractional availability as a function of voltage. Solid lines correspond to WT NaV1.2 alone (black) and NaV1.2+FGF14A (red), while symbols show values for FGF14A expressed with NaV1.2_IQM (solid symbols). Dashed line is fitted Boltzmann function for the IQM+FGF14A points. (B–D) Similar comparisons for FGF13A (B), FGF12A (C), and FGF11A (D). (E) Mean ± SD and individual values for Vh estimates from Boltzmann fits from A–D with the indicated number of cells in each case given below. (F) Comparison of effective valence, z, of SSI for each NaV1.2_IQM+FGF-A homologue. Statistical comparisons are in Table S4. Refer to the image caption for details.$ Panel A shows a steady state inactivation curve with y-axis fractional availability and x-axis command potential in millivolts, including WT Nav1.2 alone (black line), Nav1.2 plus FGF14A (red line), and IQM plus FGF14A (red symbols with dashed fit). Panel B shows a steady state inactivation curve with y-axis fractional availability and x-axis command potential in millivolts, including WT Nav1.2 alone (black line), Nav1.2 plus FGF13A (blue line), and IQM plus FGF13A (blue symbols with dashed fit). Panel C shows a steady state inactivation curve with y-axis fractional availability and x-axis command potential in millivolts, including WT Nav1.2 alone (black line), Nav1.2 plus FGF12A (magenta line), and IQM plus FGF12A (magenta symbols with dashed fit). Panel D shows a steady state inactivation curve with y-axis fractional availability and x-axis command potential in millivolts, including WT Nav1.2 alone (black line), Nav1.2 plus FGF11A (green line), and IQM plus FGF11A (green symbols with dashed fit). Panel E shows a scatter plot with y-axis Vh in millivolts and x-axis conditions, including individual data points with mean and standard deviation. Panel F shows a scatter plot with y-axis z value and x-axis conditions, including individual data points with mean and standard deviation.

SSI behavior for Na _V 1.2_IQM expressed with different FGF-A homologues. (A) SSI protocols (identical to those in Fig. 2) were used to examine fractional availability as a function of voltage. Solid lines correspond to WT Na_V1.2 alone (black) and Na_V1.2+FGF14A (red), while symbols show values for FGF14A expressed with Na_V1.2_IQM (solid symbols). Dashed line is fitted Boltzmann function for the IQM+FGF14A points. (B–D) Similar comparisons for FGF13A (B), FGF12A (C), and FGF11A (D). (E) Mean ± SD and individual values for V_h estimates from Boltzmann fits from A–D with the indicated number of cells in each case given below. (F) Comparison of effective valence, z, of SSI for each Na_V1.2_IQM+FGF-A homologue. Statistical comparisons are in Table S4.