Panel a shows a map of Colombia with various colored markers indicating the geographic distribution of CGD cases. The markers are color-coded to represent different genetic diagnoses: CYBB, CYBA, NCF1, NCF2, NCF4, and no genetic diagnosis. Panel b is a vertical bar graph showing DHR oxidation levels in Colombian CGD patients, with the y-axis labeled DHR Stimulation index (SI) and the x-axis labeled with different patient groups. Panel c is a line graph depicting neutrophil superoxide generation over a time course of 60 minutes in response to serum-opsonized heat-killed Candida albicans (HKC.alb) for controls and patient P14. The y-axis is labeled RLU (x 10 superscript 3) and the x-axis is labeled Time (min). Panel d is a line graph showing the same measurement in response to heat-killed Histoplasma capsulatum (HKH.cap). Panel e is a line graph showing the response to serum-opsonized zymosan (SOZ). Panel f is a line graph showing the response to PMA. Each graph includes error bars indicating standard deviation.
Geographic distribution and impaired NADPH oxidase activity in Colombian patients with CGD. (a) Geographic distribution of CGD cases in Colombia. (b) DHR oxidation levels in Colombian CGD patients (gp91phox, n = 13; p22phox, n = 2; p40phox, n = 2; p47phox, n = 1; and p67phox, n = 1), including the preserved PMA-induced activity of neutrophils seen in neutrophils of two patients with p40phox deficiency. (c–f) In patient P14 with the p40phox deficiency, neutrophil superoxide generation was measured over a time course of 60 min in response to serum-opsonized heat-killed C. albicans (HKC.alb) (c), heat-killed H. capsulatum (HKH.cap) (d), serum-opsonized zymosan (SOZ) (e), and PMA (f). The errors bars indicate standard deviation (SD) in panels b–e. HC, healthy control; NS, non stimulated.