Figure S5.
The image contains two panels, A and B, each showing immunofluorescence staining of cells. Panel A displays staining for G 3 B P 1, while Panel B displays staining for G 3 B P 2. Each panel compares wild-type (W T) cells and D B R 1 knockout (K O) cells, both with and without poly I:C treatment, as indicated by minus and plus signs. The merged images show the combined staining of G 3 B P 1 or G 3 B P 2 (red), D B R 1 (green), and D A P I (blue) for nuclear staining. Individual channels for G 3 B P 1 or G 3 B P 2, D B R 1, and D A P I are also shown separately beneath the merge. In W T cells without poly I:C, G 3 B P 1 and G 3 B P 2 show mostly diffuse cytoplasmic staining. Upon poly I:C treatment, red puncta appear, indicating stress granule formation. In D B R 1 K O cells, G 3 B P 1 and G 3 B P 2 puncta are more prominent, particularly after poly I:C treatment, suggesting enhanced stress granule formation. D B R 1 staining is present in W T cells and reduced or absent in D B R 1 K O cells. D A P I staining marks nuclei in all conditions. A scale bar is shown in the bottom right corner of each panel.
Stress granule formation in WT and DBR1 KO cells. (A and B) IF staining of G3BP1 (A) and G3BP2 (B) in WT and DBR1 KO cells. Data are representative of at least three independent experiments. The scale bar represents 10 µm.