Figure S3.
The image presents a Western blot analysis showing the levels of various proteins over a 10 day time course following s i R N A mediated knockdown of D B R 1. The blot includes lanes for negative control s i R N A and D B R 1 s i R N A at 2 days, 4 days, and 10 days, each shown with and without poly I:C treatment. Molecular weight markers in kilodaltons are indicated at 75 and 37. The proteins analyzed are P-P K R, P K R, P-e I F 2 alpha, e I F 2 alpha, beta-Actin, D B R 1, and G A P D H. In the negative control s i R N A condition, poly I:C treatment increases phosphorylation of P K R and e I F 2 alpha. In the D B R 1 s i R N A conditions, D B R 1 protein levels progressively decrease from 2 days to 10 days, confirming knockdown efficiency. Correspondingly, poly I:C induced phosphorylation of P K R and e I F 2 alpha appears reduced compared to the negative control, particularly at later time points when DBR1 levels are lowest. Total P K R and total e I F 2 alpha remain relatively stable across conditions. Beta-Actin and G A P D H serve as loading controls and show consistent band intensity. The blot shows temporal changes in protein levels at different time points, indicating the impact of D B R 1 depletion on P K R activation and downstream e I F 2 alpha phosphorylation in response to poly I:C.
Time course depletion of DBR1 reveals that PKR activation correlates with DBR1 protein levels. Western blot analysis showing DBR1 protein levels following siRNA-mediated knockdown over a 10-day time course. Corresponding levels of total and phosphorylated PKR and eIF2α (P-PKR and P-eIF2α) were examined at the indicated time points. Data are representative of three independent experiments. Source data are available for this figure: SourceData FS3.