Figure S1.
The vertical bar graph compares human lariat reads per one million mapped reads across different genotypes (D B R 1 plus slash plus, D B R 1 I 120 T slash I 120 T, and D B R 1 Y 17 H slash Y 17 H) upon I A V infection. The x-axis represents the infection conditions: N I, 8 h, and 24 h. The y-axis represents the number of human lariat reads per one million mapped reads. There are three grouped sets of vertical bars, each corresponding to a different genotype. Each group contains three gray bars representing N I, 8 h, and 24 h. Error bars are shown on each bar, indicating variability. For the D B R 1 plus slash plus genotype, the bars show no significant change across N I, 8 h, and 24 h, as indicated by n s. For the D B R 1 I 120 T slash I 120 T genotype, there is a significant increase at 24 h compared with N I, indicated by an asterisk. The 8 h bar is higher than N I but not marked as significant. For the D B R 1 Y 17 H slash Y 17 H genotype, there is a significant increase at 24 h compared with N I, indicated by a double asterisk. The 8 h value is increased relative to N I but is not marked with a significance symbol. Statistical annotations shown are n s, asterisk, and double asterisk. The bracket lines above bars indicate the specific comparisons. The x-axis label I A V infection is centered below the grouped conditions. Values are approximate based on visual inspection of the bar heights.
Cellular lariats increase in DBR1 mutant cells upon influenza virus infection. Human lariat levels in RNA-seq data from WT (DBR1 +/+) and DBR1 mutant (DBR1 I120T/I120T and DBR1 Y17H/Y17H) patient cell lines that were not infected (NI), infected with influenza virus (IAV) for 8 h, and infected with IAV for 24 h (P value from two-sided t test; ns, not significant; *P < 0.05, **P < 0.01).