Figure S3.
Steady-state inactivation of Kv1.3 in the presence of KCNE4 variants. HEK293 cells were transfected with Kv1.3 in the absence or presence of different KCNE4 variants (+145D, +145E, +145A). (A) Representative two-step currents analyzing the steady-state inactivation of Kv1.3. The cells were held at −80 mV. Currents were elicited by a 2-s depolarizing prepulse from −60 mV to +40 mV followed by a 250-ms test pulse at +40 mV to measure the voltage dependence of inactivation. (B) Relativized peak currents of the test pulse were fitted to a Boltzmann equation. Black circles, Kv1.3 alone; blue squares, +145D; orange triangles, +145E; and green diamonds, +145A. The values represent the mean ± SE of 2–3 cells. Refer to the image caption for details. Panel A: Four line graphs showing representative 2-step currents, with the x-axis representing time in milliseconds, and the y-axis represents normalized current. The graphs are labeled as Kv1.3, plus 145D, plus 145E, and plus 145A. Panel B: A line graph showing relativized peak currents of the test pulse fitted to a Boltzmann equation. The x-axis represents voltage in millivolts, and the y-axis represents the ratio of current to maximum current.

Steady-state inactivation of Kv1.3 in the presence of KCNE4 variants. HEK293 cells were transfected with Kv1.3 in the absence or presence of different KCNE4 variants (+145D, +145E, +145A). (A) Representative two-step currents analyzing the steady-state inactivation of Kv1.3. The cells were held at −80 mV. Currents were elicited by a 2-s depolarizing prepulse from −60 mV to +40 mV followed by a 250-ms test pulse at +40 mV to measure the voltage dependence of inactivation. (B) Relativized peak currents of the test pulse were fitted to a Boltzmann equation. Black circles, Kv1.3 alone; blue squares, +145D; orange triangles, +145E; and green diamonds, +145A. The values represent the mean ± SE of 2–3 cells.

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