Figure S1.
Panel A: Confocal images of HEK293 cells transfected with KCNE4-CFP variants and a DsRed membrane marker (red). The images show the membrane, KCNE4, and merged views. The right panels display pixel-by-pixel intensity profiles indicated by white arrows in the merged images. Panel B: A bar graph showing that the x-axis represents the KCNE4 variants 145D, 145E, and 145A, and the y-axis represents the MOC values. The graph includes individual values and the mean with standard error for each variant. Statistical analysis indicates no significant difference among the variants.
Plasma membrane trafficking of the KCNE4 variants. (A) Representative confocal images of HEK293 cells transfected with KCNE4-CFP 145D, 145E, and 145A (blue) and a DsRed membrane marker (red). The panels on the right show the respective pixel-by-pixel profiles indicated by the white arrows in the merged panels. Blue, KCNE4 variants; red, membrane marker. (B) MOC for KCNE4 overlapping with the membrane marker (Mb). The individual values and the mean ± SE were plotted for KCNE4 145D (37 cells), 145E (55 cells), and 145A (25 cells). Statistical analysis: one-way ANOVA. P: 0.225, n.s., not significant.