![KCNE4 145D/E polymorphism differentially decreases Kv1.3 currents. HEK293 cells were transfected with Kv1.3 in the absence or presence (+) of different variants of KCNE4 (+145D, +145E, and +145A). (A) Representative currents of Kv1.3. Currents were elicited by 250-ms-long pulses from −80 mV to +60 mV in 10-mV steps and tail currents measured at −40 mV. (B) Peak current density was measured at +60 mV for Kv1.3 either without (1,245 ± 133 pA/pF, n = 10) or with different KCNE4 mutants (+145D [732 ± 133 pA/pF, n = 13], +145E [266 ± 32 pA/pF, n = 19], and +145A [290 ± 66 pA/pF, n = 8]). Statistical analysis: Welch’s ANOVA test (P < 0.0001) and unpaired t test with Welch’s correction. Specific P values were as follows: *P < 0.05 (0.0123, Kv1.3 vs. +145D); **P < 0.01 (0.0038, +145D vs. +145E; 0.0077, +145D vs. +145A); ***P < 0.001 (<0.0001, Kv1.3 vs. +145E; <0.0001, Kv1.3 vs. +145A). (C) IV relationship for Kv1.3 currents in the absence or presence of different KCNE4 variants. (D) GV plot of Kv1.3 tail currents measured at −40 mV. Black circles, Kv1.3 alone; blue squares, +145D; orange triangles, +145E; and green diamonds, +145A. The values represent the mean ± SE of 5–8 independent cells for each condition in 5–8 experiments. Recordings were obtained from multiple independent transfections as follows: Kv1.3 (8), +145D (8), +145E (11), and +145A (6). In each experiment, two different random conditions were recorded. GV plot, conductance–voltage plot. n, number of cells. Refer to the image caption for details.](https://cdn.rupress.org/rup/content_public/journal/jgp/158/4/10.1085_jgp.202513936/1/jgp_202513936_fig2.png?Expires=1782544186&Signature=4JHJ0f-wv0t4dletgtlmCJ2~gP2O0wZMAOC3neaRD5FEj5fDtRaGZD-ISh~su-IY8IY32CnjwTkBl23EbDF4dSTMdd71XOPAHO9ul2k5z-J4j6MDyKQkoDg7S70yk3V7Hnm8GF9~pV-pfoSk29Kz8o0D00oZ4leTab801gjTe2RE0Q0JNCHVlo5t5rZyJKYG-V6fyz~da7XMWJTDnyFfjvkYuds-DleRQWFL2KL29cXP--AQ-12h3el6JtAxBqu0oLIL1hMawVamUhs33JdP8Qe2gW8j8OHsvwYupJfUgJKe5MwSGZ6GQOGzXc~6Hoy6mllWEwE-g3NloNCzMBtzTg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Panel A: The representative currents of Kv1.3 and its variants. The x-axis represents time in milliseconds, and the y-axis represents current in picoamperes per picofarad. Panel B: A bar graph comparing peak current density at plus 60 millivolts for Kv1.3 and its variants. The x-axis lists the conditions (Kv1.3, plus 145D, plus 145E, plus 145A), and the y-axis represents current density in picoamperes per picofarad. Panel C: Line graph showing the IV relationship for Kv1.3 currents in the absence or presence of different KCNE4 variants. The x-axis represents voltage in millivolts, and the y-axis represents current in picoamperes per picofarad. Panel D: Line graph showing the GV plot of Kv1.3 tail currents measured at 40 millivolts. The x-axis represents voltage in millivolts, and the y-axis represents normalized conductance.
KCNE4 145D/E polymorphism differentially decreases Kv1.3 currents. HEK293 cells were transfected with Kv1.3 in the absence or presence (+) of different variants of KCNE4 (+145D, +145E, and +145A). (A) Representative currents of Kv1.3. Currents were elicited by 250-ms-long pulses from −80 mV to +60 mV in 10-mV steps and tail currents measured at −40 mV. (B) Peak current density was measured at +60 mV for Kv1.3 either without (1,245 ± 133 pA/pF, n = 10) or with different KCNE4 mutants (+145D [732 ± 133 pA/pF, n = 13], +145E [266 ± 32 pA/pF, n = 19], and +145A [290 ± 66 pA/pF, n = 8]). Statistical analysis: Welch’s ANOVA test (P < 0.0001) and unpaired t test with Welch’s correction. Specific P values were as follows: *P < 0.05 (0.0123, Kv1.3 vs. +145D); **P < 0.01 (0.0038, +145D vs. +145E; 0.0077, +145D vs. +145A); ***P < 0.001 (<0.0001, Kv1.3 vs. +145E; <0.0001, Kv1.3 vs. +145A). (C) IV relationship for Kv1.3 currents in the absence or presence of different KCNE4 variants. (D) GV plot of Kv1.3 tail currents measured at −40 mV. Black circles, Kv1.3 alone; blue squares, +145D; orange triangles, +145E; and green diamonds, +145A. The values represent the mean ± SE of 5–8 independent cells for each condition in 5–8 experiments. Recordings were obtained from multiple independent transfections as follows: Kv1.3 (8), +145D (8), +145E (11), and +145A (6). In each experiment, two different random conditions were recorded. GV plot, conductance–voltage plot. n, number of cells.