Figure S1.
Panel A: A grayscale image of an astrocyte with labeled sub-regions: branches, branchlets, and endfeet. The scale bar represents 10 micrometers. Panel B: Five dot plots showing the amplitude of Ca2+ positive signals (dF/F) under resting and illuminated conditions for five different cells. The horizontal axis categorizes the data into branches, branchlets, and endfeet, while the vertical axis represents the amplitude (dF/F). Each dot represents a data point, and error bars indicate the mean standard error of the mean (SEM). The plots show variations in amplitude across different sub-regions and conditions.
Light-induced Ca 2+ signal amplitude dynamics. (A) Composite Ca2+ signals in astrocyte subregions (branches, branchlets, endfeet) over 250 s. (B) AQuA software analysis of Ca2+ amplitude under resting (−) vs. illuminated (+) conditions over 250 s. Error bars represent the mean ± SEM, n = 5 independent experiments. Scale bars: 10 μm.