Figure S2.
CD169+ macrophages suppress tumour growth independent of acquired immunity. (A) Gating strategy of immune cells in B16-F10–bearing skin (representative of five mice). (B–D) The numbers of DCs, neutrophils, natural killer (NK) cells, CD8+ T cells, cytotoxic CD8+ T cells, CD4+ T cells, Foxp3+ CD4+ T cells, and B cells in the tumors (day 7–11) with isotype mAb or anti-CSF1R mAb treatment (n = 5, data are representative of three independent experiments. Each circle represents one mouse). P value was calculated using Student’s t test. (E) The tumor volumes of B16-F10 melanoma with isotype mAb versus anti-CSF1R mAb in RAG1 KO mice (n = 10, data are combined from two independent experiments with five mice per group, each circle represents one mouse). P value was calculated using Student’s t test. Refer to the image caption for details. Panel A shows flow cytometry plots illustrating gating strategies for various immune cells in B16-F10-bearing skin, including neutrophils, dendritic cells, regulatory T cells, CD8 positive T cells, cytotoxic CD8 positive T cells, B cells, and natural killer cells. Panel B shows bar graphs comparing cell counts of dendritic cells, neutrophils, and natural killer cells in tumors treated with isotype mAb or anti-CSF1R mAb, with treatment type on the x-axis and cell counts per 10 mm punch biopsy on the y-axis. Panel C shows bar graphs comparing cell counts of CD8 positive T cells, cytotoxic CD8 positive T cells, and IFNγ positive CD8 positive T cells in tumors treated with isotype mAb or anti-CSF1R mAb, with treatment type on the x-axis and cell counts per 10 mm punch biopsy on the y-axis. Panel D shows bar graphs comparing cell counts of CD4 positive T cells, Foxp3 positive CD4 positive T cells, and B cells in tumors treated with isotype mAb or anti-CSF1R mAb, with treatment type on the x-axis and cell counts per 10 mm punch biopsy on the y-axis. Panel E shows a bar graph comparing tumor volumes of B16-F10 melanoma in RAG1 KO mice treated with isotype mAb or anti-CSF1R mAb, with treatment type on the x-axis and tumor volume in cubic millimeters on the y-axis.

CD169+ macrophages suppress tumour growth independent of acquired immunity . (A) Gating strategy of immune cells in B16-F10–bearing skin (representative of five mice). (B–D) The numbers of DCs, neutrophils, natural killer (NK) cells, CD8+ T cells, cytotoxic CD8+ T cells, CD4+ T cells, Foxp3+ CD4+ T cells, and B cells in the tumors (day 7–11) with isotype mAb or anti-CSF1R mAb treatment (n = 5, data are representative of three independent experiments. Each circle represents one mouse). P value was calculated using Student’s t test. (E) The tumor volumes of B16-F10 melanoma with isotype mAb versus anti-CSF1R mAb in RAG1 KO mice (n = 10, data are combined from two independent experiments with five mice per group, each circle represents one mouse). P value was calculated using Student’s t test.

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