Panel A: Confocal microscopy images showing wing disc with f z r overexpression clones labeled by G F P and stained with c D c p 1 and D A P I indicating apoptotic cell engulfment. Panel B: Confocal microscopy images showing wing disc with r u x overexpression clones labeled by G F P and stained with c D c p 1 and D A P I indicating apoptotic cell engulfment. Panel C: Confocal microscopy images showing 18 G y irradiated wing disc with f z r overexpression clones labeled by G F P and stained with c D c p 1 and D A P I. Panel D: Confocal microscopy images showing 18 G y irradiated wing disc with r u x overexpression clones labeled by G F P and stained with c D c p 1 and D A P I. Panel E: Confocal microscopy images showing wing disc with f z r overexpression clones stained with D A P I including zoomed and orthogonal views. Panel F: Confocal microscopy images showing wing disc with r u x overexpression clones stained with D A P I including zoomed and orthogonal views. Panel G: Confocal microscopy images showing 18 G y irradiated wing disc with f z r overexpression clones stained with D A P I highlighting engulfment of neighboring cells. Panel H: Confocal microscopy images showing 18 G y irradiated wing disc with r u x overexpression clones stained with D A P I highlighting engulfment of neighboring cells. Panel I: Confocal microscopy images showing wing discs with control, f z r, and r u x overexpression clones stained with D A P I and c D c p 1. Panel J: Bar graph quantifying number of c D c p 1 positive cells across control, f z r, and r u x conditions. Panel K: Confocal microscopy images showing wing disc with f z r overexpression clones induced by p t c drives G F P in m a h j 1 background stained with c D c p 1 and D A P I with engulfment events. Panel L: Confocal microscopy images showing wing disc with f z r overexpression clones induced by p t c drives G F P in m a h j 1 background stained with M m p 1 and D A P I indicating J N K activation. Panel M: Time lapse microscopy images showing f z r overexpression clones induced by p t c drives G F P in m a h j 1 background with protrusions contacting neighboring cells. Panel N: Confocal microscopy images showing wing disc with f z r overexpression clones induced by a c t double arrow G F P stained with D A P I and N i m C 1 indicating hemocyte interaction.
Induced polyploid cells exhibit active engulfment. (A and B) Confocal images of fzr-OE (A, wing disc) and rux-OE (B, eye disc) clones stained with cDcp1 (red) and DAPI (blue). (C and D) Confocal images of fzr-OE (C) and rux-OE (D) clones after 18 Gy γ-ray exposure, stained with cDcp1 (red) and DAPI (blue). White arrowheads indicate apoptotic (red) cells engulfed by green polyploid cells. Scale bars: 20 μm. (E and F) Single-plane confocal images of fzr-OE (E) and rux-OE (F) clones stained with DAPI (magenta). (E′ and F′) Zoomed-in views of white dashed rectangles in E and F, respectively. (E″ and F″) Orthogonal views of E’ and F’. (G and H) Single-plane confocal images of fzr-OE (G) and rux-OE (H) after 18 Gy γ-ray treatment. Stained with DAPI (magenta). (G′ and H′) Zoomed-in views of white dashed rectangles in G and H, respectively. (G″ and H″) Orthogonal views of G′ and H′. Yellow arrowheads indicate live cells engulfed by green polyploid cells. Scale bars: 20 μm. (I) Confocal images of Act>>GFP + control (Ctrl, +), fzr-OE, or rux-OE discs stained with DAPI (blue) and cDcp1 (red). (J) Quantification of the number of cDcp1-positive (cDcp1+) cells in ctrl (n = 15), fzr-OE (n = 10) and rux-OE (n = 4) groups. Scale bars: 20 μm. (K) Imaginal wing discs of fzr-OE induced by ptc>GFP (green) in a mahj1 (red) background, stained with cDcp1 (gray) and DAPI (blue). Yellow arrows indicate cells engulfed by fzr-OE cells. Scale bars: 20 μm. (L) Imaginal wing discs of fzr-OE induced by ptc>GFP (green) in a mahj1 (red) background, stained with Mmp1 (gray) and DAPI (blue). White dashed rectangle highlights fzr-OE + mahj1 cells showing JNK activation, marked by Mmp1 staining (gray). Scale bars: 20 μm. (M) Time-lapse images of fzr-OE induced by ptc>GFP (green) in a mahj1 (red) background. Yellow arrowheads indicate green cell protrusions contacting red cells. Scale bars: 20 μm. (N) Confocal images of imaginal wing discs with fzr-OE induced by act>>GFP (green), stained with DAPI (gray), NimC1 (red). Green arrowheads indicate fzr-OE cells; red arrowheads indicate hemocytes. Scale bar: 20 μm.