Figure S3.
Panel A shows a sequence of amino acids with predicted cleavage sites for different protease families. Cleavage sites are color-coded: black for Aspartic protease, green for Cysteine protease, yellow for Metalloprotease, blue for Serine protease, and red for multiple protease superfamilies. Panel B is a bar graph showing endothelial cortactin mean fluorescence intensity (MFI) under medium and TNFα conditions. The y-axis represents endothelial cortactin MFI, and the x-axis shows the conditions. The bars indicate no significant difference between the conditions. Panel C is a representative image of neutrophils interacting with HUVEC under flow conditions, with a scale bar of 200 micrometers. Panel D is a bar graph showing endothelial cortactin MFI under different conditions: TNFα with neutrophils, and TNFα with whole blood. The y-axis represents endothelial cortactin MFI, and the x-axis shows the conditions. The graph indicates no further reduction in cortactin levels with whole blood compared to isolated neutrophils.
Cortactin is a target for NSP. (A) Prediction of putative cleavage sites for different protease families in the cortactin sequence using PROSPER software. (B) TNFα alone does not induce loss of cortactin under flow conditions; n = 4. (C) Representative image of neutrophils interacting with HUVEC under flow; n = 4; scale bar = 200 µm. (D) HUVEC perfusion under flow conditions with whole blood does not further reduce cortactin levels compared with HUVEC perfusion with isolated neutrophils; n = 4.