Panel A: An illustration shows the identification of antigen specificity. It includes a depiction of CD 8 cells interacting with antigens and labeled with biotin. Single-cell RNA sequencing (scRNA-seq) and single-cell TCR sequencing (scTCR-seq) are illustrated with colored bars representing different gene segments. Panel B: A UMAP plot shows the phenotype and TCR potency. The plot is labeled with UMAP_1 and UMAP_2 axes and shows clusters of cells with different TCR potency and exhaustion scores. Panel C: A diagram illustrates the Ly49 plus regulatory state. It shows immune checkpoints, NK receptors, SPP1, granzyme C, and Ly49 genes interacting with a cell. Panel D: A diagram depicting the mechanism of discovery of alpha PD-1 therapy. It shows different types of T cells (T subscript EM, T subscript EFF, T subscript EX, T subscript Ly49) and their responses to alpha PD-1 therapy. Panel E: A diagram predicts clinical response. It shows two figures representing responders and non-responders based on the levels of T subscript EM, T subscript EFF, and T subscript Ly49/KIR cells.