Figure S4.
Time-lapse bright-field imaging of WT and VRAC KO BMDMs in hypo-osmotic media. Representative images of WT and VRAC KO BMDM incubated in iso- or hypo-osmotic media. BMDMs were placed immediately in iso- or hypo-osmotic media immediately before commencing time-lapse microscopy (n = 5) (scale = 25 µm). Refer to the image caption for details. The images are arranged in a grid with four rows and six columns. Each row represents a different condition: WT in iso-osmotic media, KO in iso-osmotic media, WT in hypo-osmotic media, and KO in hypo-osmotic media. Each column represents a different time point: 1 minute, 15 minutes, 60 minutes, 120 minutes, 180 minutes, and 240 minutes. The images show the morphological changes of the BMDMs over time under different osmotic conditions. The scale bar in each image represents 25 micrometers.

Time-lapse bright-field imaging of WT and VRAC KO BMDMs in hypo-osmotic media. Representative images of WT and VRAC KO BMDM incubated in iso- or hypo-osmotic media. BMDMs were placed immediately in iso- or hypo-osmotic media immediately before commencing time-lapse microscopy (n = 5) (scale = 25 µm).

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