Figure S3.
Panel A: A scatter bar plot comparing Ifnb fold expression in WT and VRAC KO BMDMs under different conditions. The horizontal axis represents different treatments: control, hypo-osmotic media, and hypo-osmotic media with NU7441. The vertical axis represents Ifnb fold expression. WT and VRAC KO are represented by open and filled circles, respectively. Panel B: A scatter bar plot comparing Cxcl10 fold expression in WT and VRAC KO BMDMs under different conditions. The horizontal axis represents different treatments: control, hypo-osmotic media, and hypo-osmotic media with NU7441. The vertical axis represents Cxcl10 fold expression. WT and VRAC KO are represented by open and filled circles, respectively. Panel C: A scatter bar plot showing IFN release in supernatant from WT BMDMs stimulated with poly dA:dT in the presence of different inhibitors or vehicle control. The horizontal axis represents different treatments: vehicle, H151, A151, Ru.251, and NU7441. The vertical axis represents IFN release in ng per mL.
Related to Fig. 3: changes in cell volume drive IFNβ responses through a DNA- and TBK1-dependent pathway. (A and B) qRT-PCR analysis of Ifnb (A) and Cxcl10 (B) in WT and VRAC KO BMDMs incubated in hypo-osmotic media (50% H2O vol/vol in DMEM) for 6 h in the presence of Nu7441 (100 nM) or vehicle control (DMSO 0.5% vol/vol) (n = 3). (C) IFNβ release in supernatant from WT BMDMs stimulated with transfected poly dA:dT (1 µg/ml) in the presence of H151 (10 µM), A151 (1 µM), Ru.251 (10 µM), NU7441 (100 nM), or vehicle control (DMSO, 0.5% vol/vol) for 6 h (n = 3). Values shown are the mean ± the SEM.