Figure S2.
Panel A: A scatter bar plot comparing the fold expression of Il18 in WT and VRAC KO BMDMs under iso- and hypo-osmotic conditions. The x-axis is labeled Hypo with categories minus and plus, and the y-axis is labeled Il18 (Fold expression). WT and VRAC KO BMDMs are represented by open and filled circles, respectively. The graph shows higher expression in VRAC KO BMDMs under hypo-osmotic conditions. Panel B: A scatter bar plot comparing the fold expression of Ifna in WT and VRAC KO BMDMs under iso- and hypo-osmotic conditions. The x-axis is labeled Hypo with categories minus and plus, and the y-axis is labeled Ifna (Fold expression). WT and VRAC KO BMDMs are represented by open and filled circles, respectively. The graph shows higher expression in VRAC KO BMDMs under hypo-osmotic conditions. Panel C: A scatter bar plot comparing the fold expression of Tnf in WT and VRAC KO BMDMs under iso- and hypo-osmotic conditions. The x-axis is labeled Hypo with categories minus and plus, and the y-axis is labeled Tnf (Fold expression). WT and VRAC KO BMDMs are represented by open and filled circles, respectively. The graph shows higher expression in VRAC KO BMDMs under hypo-osmotic conditions. Panel D: A western blot showing viperin and beta-actin levels in WT and IFNAR KO BMDMs stimulated with recombinant murine IFN. The blot shows bands for viperin and beta-actin under different conditions. Panel E: A western blot showing viperin and beta-actin levels in WT BMDMs stimulated with poly I:C. The blot shows bands for viperin and beta-actin under different conditions.
Related to Fig. 2: additional cytokines influenced by cell volume and validation of IFNAR KO BMDMs. (A–C) qRT-PCR analysis of Il18 (A), pan-Ifna transcripts (B), and Tnf (C) in WT and VRAC KO BMDMs incubated in iso- or hypo-osmotic media (6 h) (n = 6). (D and E) Western blot for viperin in WT and VRAC KO BMDMs stimulated with recombinant murine IFNβ (200 U/ml) (D) or poly I:C (1 µg/ml) (E) (6 h) (n = 3). *P < 0.05, ***P < 0.001, determined by a two-way ANOVA with Dunnett’s post hoc analysis. Values shown are the mean ± the SEM.