Figure S5.
Panel A: A Western blot image showing protein levels of artificially tethered ERMES members. The blot is probed with anti-FLAG and anti-Tom70 antibodies. The lanes are labeled as vector, MMM1-3FLAG-mNG, MDM12-3FLAG-mNG, MDM34-3FLAG-mNG, MMM1-3FLAG-mNG-Fis1C, Sec66N-MDM12-3FLAG-mNG-Fis1C, and Sec66N-MDM34-3FLAG-mNG-Fis1C. Arrowheads indicate the expressed proteins, and asterisks indicate non-specific signals. Tom70 serves as the loading control. Panel B: A Western blot image showing protein levels of Mmm1-mNG-Fis1C with partially truncated Mmm1. The blot is probed with anti-FLAG and anti-Tom70 antibodies. The lanes are labeled as vector, MMM1-3FLAG-mNG-Fis1C, and various truncated forms of Mmm1. Arrowheads indicate the expressed proteins, and asterisks indicate non-specific signals. Tom70 serves as the loading control. Panel C: A Western blot image showing protein levels of tethering constructs with different transmembrane and SMP domains. The blot is probed with anti-mNG and anti-Tom70 antibodies. The lanes are labeled as vector, ADH1p-Sec66N-SMP-mNG-Fis1C, and ADH1p-Tom70N-SMP-mNG-Ubc6C, with various constructs of Mmm1, Mdm12, and Mdm34. Arrowheads indicate the expressed proteins, and asterisks indicate non-specific signals. Tom70 serves as the loading control.
Protein levels of the artificially tethered proteins used in this study. (A–C) Total cell lysates were analyzed by immunoblotting. Arrowheads indicate the expressed proteins. Asterisks indicate nonspecific signals. Tom70 was used as loading controls. (A) Protein levels of artificially tethered ERMES members. (B) Protein levels of Mmm1-mNG-Fis1C with partially truncated Mmm1. (C) Protein levels of tethering constructs with different transmembrane and SMP domains. Source data are available for this figure: SourceData FS5.