Figure S3.
Panel A: Tetrad analysis of sporulated MDM12/mdm12 delta MDM34/mdm34 delta diploids expressing the ChiMERA tether. Segregants are color-coded by genotype: wild-type (green), mdm12 delta (pink), mdm34 delta (yellow), and the mdm12 delta mdm34 delta double mutant (blue). Panel B: Representative tetrads illustrating the impact of Mmm1 overexpression (GPD-MMM1) on the vegetative growth of mdm12 delta mutants. Genotypes are indicated by color: wild-type (green), GPD-MMM1 (pink), mdm12 delta (yellow), and mdm12 delta GPD-MMM1 (blue). Panel C: Assessment of progeny viability via tetrad analysis following Mmm1 overexpression in the mdm12 delta vps13 delta background. Colors denote wild-type (green), vps13 delta (pink), mdm12 delta (yellow), and mdm12 delta vps13 delta (blue). Panel D: Predicted Aligned Error (PAE) plot for the AlphaFold structural model. The matrix displays error values in Ångströms, with a color-coded scale ranging from 0 to 30 Ångströms to indicate local alignment confidence.
Tetrad analysis of ChiMERA in ERMES deletion yeast. (A) Representative tetrads from the sporulation of MDM12/mdm12Δ MDM34/mdm34Δ diploids expressing ChiMERA. (B) Tetrad analysis of the effect of Mmm1 overexpression (with an integrated GPD promoter on the endogenous gene) on growth of mdm12Δ. (C) Tetrad analysis of the effect of Mmm1 overexpression (with GPD promoter on a 2μ plasmid) on growth of mdm12Δ/vps13Δ. (D) Predicted aligned error plot for the AlphaFold model in Fig. 3 B.