Figure S2.
From left to right, the columns show mtBFP, Vps13 superscript GFP, mCherry-SKL, and Vps13 slash perox. The top four rows depict cells under GPDpr-MCP1 conditions, showing distinct green Vps13 superscript GFP puncta that occasionally overlap with the cyan mitochondrial networks but remain largely separate from the peroxisome dots. The bottom four rows represent the gem1 delta GPDpr-MCP1 strain, where the green Vps13 superscript GFP signal appears more diffuse or expanded along the mitochondrial structures compared to the control. In the merged far-right column, the Vps13 signal and magenta peroxisomal signal are shown together to evaluate spatial proximity, with small white scale bars in the bottom right corner of each merged frame denoting 2 micrometers.
Effect of Gem1 deletion on Vps13 localization. Multiple representative images of Vps13^GFP subcellular localization in comparison with mitochondria (mtBFP) and peroxisomes (mCherry-SKL) in Mcp1 overexpression conditions, both with and without Gem1. Scale bars are 2 μm.