Figure S1.
Panel A: Comparison of transposon insertion profiles for MCP1 and YPT35 across wild-type, vps13-D716H, and mmm1 delta vps13-D716H backgrounds. The maps illustrate divergent mutational landscapes and insertion densities between these genetic contexts. Panel B: Fluorescence micrographs depicting Vps13 superscript GFP recruitment to vCLAMPs (vacuole-mitochondria patches) during Vps39 and Mcp1 co-overexpression. The images evaluate the dependency of this localization on Ypt35, using mtBFP as a mitochondrial reference. Panel C: Representative images illustrating the shift in Vps13 superscript GFP subcellular distribution in response to Mcp1 overexpression. Panel D: Sequential Z-stack sections showing the distinct architecture of Vps13 superscript GFP foci in stationary phase yeast cells. Panel E: Localization of Vps13 superscript GFP relative to the mitochondrial marker Tom70-mScarlet in control versus Mcp1-overexpressing strains. Scale bars represent 2 micrometers.
Vps13 forms puncta on mitochondria. (A) Comparison of transposon density maps for MCP1 and YPT35 in wild-type, VPS13D716H, and mmm1Δ VPS13D716H yeast using saturated transposon analysis in yeast (SATAY) (Michel et al., 2017). (B) Representative images of Vps13^GFP localization to vCLAMPs in Vps39 and Mcp1 co-overexpression conditions, both with and without Ypt35. mtBFP is a mitochondrial marker. (C) Representative images of Vps13^GFP both with and without the overexpression of MCP1. (D) Individual Z planes of Vps13^GFP when cells are in stationary phase. (E) Representative images of Vps13^GFP and Tom70-mScarlet in control and Mcp1-overexpressing yeast. Scale bars are 2 μm. vCLAMPs, vacuolar and mitochondrial patches.