Figure 2.
Panel A: Microscopy images showing cells with different expressions. The left column shows bright-field images, and the right column shows EGFP/DAPI stained images. The top row shows control cells, the middle row shows cells expressing EGFP-RAB27A(WT), and the bottom row shows cells expressing EGFP-RAB27A(Trp73Arg). Cells with perinuclear aggregation are outlined with broken lines. Scale bars represent 20 micrometers. Panel B: A bar graph comparing the percentages of cells. The horizontal axis lists three categories: Control, RAB27A(WT), and RAB27A(W73R). The vertical axis represents the percentage distribution of melanosomes. The control and RAB27A(W73R) bars are near 0 percent, while the RAB27A(WT) bar is at 100 percent. Error bars represent the means plus or minus standard error of the mean (S.E.) from three independent experiments.
RAB27A(Trp73Arg) did not restore peripheral melanosome distribution in RAB27A-deficient melanocytes. (A) Typical images of melan-ash cells transiently expressing EGFP alone (control; top panels), EGFP-tagged RAB27A(WT) (middle panels), and EGFP-tagged RAB27A(Trp73Arg) (indicated as W73R; bottom panels). Cells with perinuclear aggregation were outlined with broken lines. Scale bars, 20 μm. (B) The percentages of cells showing peripheral melanosome dispersion in A. The error bars represent the means ± SE of data obtained in three independent experiments (n = 30 cells in each experiment), which were performed on different days. ***P < 0.001; NS, not significant (one-way ANOVA and Tukey’s test).