Figure S4.
Panel A shows cells with green signals clustered inside the nucleus, red signals marking expressed protein, and blue-stained nuclei. The patterns differ between control, normal protein, and mutant forms. Panel B shows similar cells with green signals in distinct nuclear regions and red signals indicating protein presence. The overlap and distribution vary across conditions. Panel C shows treated cells where the green signals appear more dispersed or altered. Red signals remain visible, and blue nuclei outline the cell shape. Differences are seen between control, normal, and mutant conditions.
WDR75 p.T46I variant colocalizes with UBF and fibrillarin. (A) U2OS cells expressing an empty vector pWPI, Flag-WDR75, or Flag-WDR75 p.T46I were stained for fibrillarin (FBL, green), anti-Flag (red), and nuclei with DAPI (blue); bars, 5 μM. (B) U2OS cells expressing an empty vector pWPI, Flag-WDR75, or Flag-WDR75 p.T46I were stained for UBF (green), anti-Flag (red), and nuclei with DAPI (blue); bars, 5 μM. (C) U2OS cells expressing a pWPI empty vector, Flag-WDR75, or Flag-WDR75 p.T46I were treated with 5 nM Act D for 4 h and then stained for fibrillarin (FBL, green), anti-Flag (red), and nuclei with DAPI (blue); bars, 5 μM. (A–C) Representative images from n = 2 independent experiments. Act D, actinomycin D.